Composite

Part:BBa_K5311020

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)


MG_010 + RNA thermometer ForU + CAP 10-3 Endolysin + His-tag

Genetic construct created to produce endolysin to induce cell death in C. acnes and E. coli. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 135
    Illegal NgoMIV site found at 546
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 294


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Categories
Parameters
None